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Unable to do a clean installation, I was upgrading my last Win2k user last week.  All went well with the exception of the fact that this week they came back to me complaining that their USB flash drive was inaccessible in My Computer.  With hardware working properly and the drive accessible in Disk Management, I was at a loss.  I finally went through an entire Windows Repair and Diagnostic Startup, individually enabling the services necessary for disk access and drive assignment.  Nothing worked until I remembered that I had removed Daemon Tools, a piece of software that assigns drive letters to ISO files, from their previous install.  Daemon Tools installs two (or three, note the wildcard) driver files in %systemroot%\system32\drivers named secdrv.sys and spdt*.sys respectively.  Removing these files and simply rebooting restored normal access and drive letter assignment to any and all mounted USB devices.